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5 gm of root, shoot and grain tissue in experiment were grinded with 80% ethanol. The crushed material was centrifuged for 10 minutes at 3000 revolution per minute (RPM). To the filtrate volumes of chloroform was added in separating funnel and in the supernatant layer amino acids was collected. Ascending Paper chromatographic technique for the separation of amino acids was followed. Whatmann No. 1 filter paper and n-butanol : acetic acid : distilled water) was taken as solvent for developing chromatogram in proportion of 4 : 1 : 5 (n-butanol : acetic acid : distilled water). The spotted paper was introduced in a chromatographic chamber and was fixed with glass rod. The lower layer of the solvent was used for running the amino acids. The spots were developed with 0.1% ninhydrin solution using automizer. Spots identified based Rf values with the standards. To study the extent of hazards under actual field conditions, certain comparative biochemical studies were carried out on our plants under field conditions that is plant growing at the far distance farm 200 mt way from road side (unpolluted = control) and at the nearest farm from the road side (polluted = 20 mt away form road side (polluted = experimental). In order to find out level of total nitrogen, analysis of soil and plant parts of three economically important plants of family Poaceae (Gramineae) done.